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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated transgelin/SM22 Polyclonal antibody

transgelin/SM22 Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-10493

Synonyms

22 kDa actin binding protein, DKFZp686P11128, Protein WS3 10, SM22, SM22 alpha, SM22alpha, SMCC, Smooth muscle protein 22 alpha, TAGLN, TAGLN1, transgelin, TRANSGELIN 1, WS3 10

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using CoraLite®594-conjugated transgelin/SM22 Polyclonal (CL594-10493)

    IF Staining of mouse heart using CL594-10493

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite®594 transgelin/SM22 antibody (CL594-10493) at dilution of 1:200.

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Tested Applications

Positive IF-P detected inmouse heart tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-10493 targets transgelin/SM22 in IF-P applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen transgelin/SM22 fusion protein Ag0764 相同性解析による交差性が予測される生物種
Full Name transgelin
Calculated molecular weight 22 kDa
Observed molecular weight 19-22 kDa
GenBank accession numberBC004927
Gene symbol transgelin/SM22
Gene ID (NCBI) 6876
RRIDAB_2919735
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The transgelin family is a group of proteins that belong to 22kd actin-related calponin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. This antibody was generated against full length transgelin 1 protein. It can cross-react with other two transgelins based on the sequence similarity.

Protocols

Product Specific Protocols
IF protocol for CL594 transgelin/SM22 antibody CL594-10493Download protocol
Standard Protocols
Click here to view our Standard Protocols