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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated human IgM Monoclonal antibody

human IgM Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2D10B10

Cat no : CL488-66484

Synonyms

IgM, IGHM, VH, MU, 2D10B10

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite® Plus 488-conjugated human IgM Monoclonal (CL488-66484)

    IF Staining of human tonsillitis using CL488-66484

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488-conjugated human IgM antibody (CL488-66484, Clone: 2D10B10 ) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of human appendicitis tissue using CoraLite® Plus 488-conjugated human IgM Monoclonal (CL488-66484)

    IF Staining of human appendicitis using CL488-66484

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CoraLite® Plus 488 human IgM antibody (CL488-66484, Clone: 2D10B10 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite® Plus 488-conjugated human IgM Monoclonal (CL488-66484)

    IF Staining of human tonsillitis using CL488-66484

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL488-66484 (IGHM antibody) at dilution of 1:100.

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Tested Applications

Positive IF-P detected inhuman appendicitis tissue, human tonsillitis tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66484 targets human IgM in WB, IF-P applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen human IgM fusion protein Ag1459 相同性解析による交差性が予測される生物種
Full Name immunoglobulin heavy constant mu
Calculated molecular weight 69 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC009851
Gene symbol IGHM
Gene ID (NCBI) 3507
RRIDAB_2883344
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 human IgM antibody CL488-66484Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Oncol Lett

Dynamin 3 overexpression suppresses the proliferation, migration and invasion of cervical cancer cells.

Authors - Jing Fa
View Article
humanWB

Rapid Commun Mass Spectrom

iTRAQ-based quantitative proteomic analysis of immune thrombocytopenia patients before and after Qishunbaolier treatment.

Authors - Burenbatu Burenbatu
View Article