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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human appendicitis tissue using CoraLite® Plus 647-conjugated human IgM Monoclonal (CL647-66484)

    IF Staining of human appendicitis using CL647-66484

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CoraLite® Plus 647 human IgM antibody (CL647-66484, Clone: 2D10B10 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite® Plus 647-conjugated human IgM Monoclonal (CL647-66484)

    IF Staining of human tonsillitis using CL647-66484

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 647 human IgM antibody (CL647-66484, Clone: 2D10B10 ) at dilution of 1:100.

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CoraLite® Plus 647-conjugated human IgM Monoclonal antibody

human IgM Monoclonal Antibody for IF-P
Cat No. CL647-66484
Clone No.2D10B10

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF-P

Synonyms

IgM, IGHM, VH, MU, 2D10B10

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 647
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF-P detected inhuman appendicitis tissue, human tonsillitis tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-66484 targets human IgM in IF-P applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen human IgM fusion protein Ag1459 相同性解析による交差性が予測される生物種
Full Name immunoglobulin heavy constant mu
Calculated molecular weight 69 kDa
GenBank accession numberBC009851
Gene Symbol IGHM
Gene ID (NCBI) 3507
RRIDAB_2920286
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IGHM is the constant region of heavy chain of IgM. IgM is the first immunoglobulin expressed during B cell development. IgM antibodies play an important role in primary immune response involved in early recognition of external invaders like bacteria and viruses, cellular waste and modified self, as well as in recognition and elimination of precancerous and cancerous lesions. The μ heavy chain disease is a rare lymphoproliferative disorder. Human immunoglobulin heavy chain locus translocations are associated with leukaemias and lymphomas, including multiple myeloma, mantle cell lymphoma, Burkitt's lymphoma and diffuse large B cell lymphoma. This antibody detects the heavy chain of human IgM (~75 kDa).

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 human IgM antibody CL647-66484Download protocol
Standard Protocols
Click here to view our Standard Protocols