Biotin-conjugated IgM Monoclonal antibody
human IgM Monoclonal Antibody for IHC
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
IHC
Conjugate
Biotin
CloneNo.
2D10B10
Cat no : Biotin-66484
Synonyms
Validation Data Gallery
Tested Applications
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
Biotin-66484 targets human IgM in IHC applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | human IgM fusion protein Ag1459 相同性解析による交差性が予測される生物種 |
| Full Name | immunoglobulin heavy constant mu |
| Calculated molecular weight | 69 kDa |
| Observed molecular weight | 75 kDa |
| GenBank accession number | BC009851 |
| Gene symbol | IGHM |
| Gene ID (NCBI) | 3507 |
| RRID | AB_2918947 |
| Conjugate | Biotin |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IGHM is the constant region of heavy chain of IgM. IgM is the first immunoglobulin expressed during B cell development. IgM antibodies play an important role in primary immune response involved in early recognition of external invaders like bacteria and viruses, cellular waste and modified self, as well as in recognition and elimination of precancerous and cancerous lesions. The μ heavy chain disease is a rare lymphoproliferative disorder. Human immunoglobulin heavy chain locus translocations are associated with leukaemias and lymphomas, including multiple myeloma, mantle cell lymphoma, Burkitt's lymphoma and diffuse large B cell lymphoma. This antibody detects the heavy chain of human IgM (~75 kDa).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Biotin human IgM antibody Biotin-66484 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
