HRP-conjugated human IgM Monoclonal antibody

human IgM Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB

Conjugate

HRP

CloneNo.

2D10B10

Cat no : HRP-66484

Synonyms

DKFZp686I15196, DKFZp686I15212, FLJ00385, human IgM, IGHM, MU, VH


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Tested Applications

Positive WB detected inhuman plasma

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

HRP-66484 targets human IgM in WB applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen human IgM fusion protein Ag1459 相同性解析による交差性が予測される生物種
Full Name immunoglobulin heavy constant mu
Calculated molecular weight 69 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC009851
Gene symbol IGHM
Gene ID (NCBI) 3507
RRIDAB_2883841
Conjugate HRP
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

This antibody detects the heavy chain of human IgM (~75 kDa). It's HRP conjugated.

Protocols

Product Specific Protocols
WB protocol for HRP human IgM antibody HRP-66484Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Mol Ther

Engineered IgM and IgG cleaving enzymes for mitigating antibody neutralization and complement activation in AAV gene transfer

Authors - Timothy J Smith