BLI binding kinetics of a mouse IgG2a anti-V5-tag antibody to a V5-tagged protein. A monoclonal mouse IgG2a anti-V5-tag antibody was immobilized using Nano-CaptureLigand mouse IgG2a, Fc-specific VHH, biotinylated on FortéBio Streptavidin (SA) Biosensors and assayed with different concentrations of a V5-tagged protein.

Immobilization of the Nano-CaptureLigand (green) on Biosensor (white) for biolayer interferometry (BLI) followed by capture of mouse IgG2a antibody (orange). The setup is ready for characterization of mouse IgG2a antibodies’ binding kinetics and affinity to substrate (red), mouse IgG2a antibody discovery and screening in crude liquids like hybridoma supernatant, serum, and plasma.

ELISA capture of mouse IgG2a antibody using Nano-CaptureLigand mouse IgG2a, Fc-specific VHH, biotinylated. 50 nM Nano-CaptureLigand mouse IgG2a, Fc-specific VHH, biotinylated was used for coating on an avidin-coated MaxiSorp plate. Mouse IgG2a antibody was titrated in a 1:2 dilution series and detected with an alkaline phosphatase-conjugated detection antibody.

Sandwich ELISA: Immobilization of the Nano-CaptureLigand on streptavidin coated microplates for ELISA. No biotinylation of the antibody is required. Coating of the streptavidin microplate with Nano-CaptureLigand (top left). Site-directed antibody immobilization via Nano-CaptureLigand (top right). Addition of sample including target (red, bottom left). Addition of detection and secondary antibody for read-out (bottom right).

Species reactivity of Secondary VHH.

PFA-fixed HeLa cells were stained with anti-GNL3 (67169-1-Ig) labeled with FlexAble CoraLite® Plus 405 Kit (KFA046, cyan) and anti-Tubulin (66240-1-Ig) labeled with FlexAble CoraLite® Plus 750 Kit (KFA044, grey). Epifluorescence images were acquired with a 20x objective and post-processed.

PFA-fixed HeLa cells were stained with anti-Actin labeled with FlexAble CoraLite® Plus 405 Kit (KFA046, cyan) and Sytox Deep Red (cell nuclei, magenta). Epifluorescence images were acquired with a 20x objective and post-processed.

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD45 (clone HI30, 65109-1-Ig) labeled with FlexAble CoraLite® Plus 750 Kit (KFA024) and anti-human CD3 (clone OKT3, 65133-1-Ig) labeled with FlexAble CoraLite® Plus 405 Kit (KFA046).

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite® Plus 750 Kit (KFA024) and anti-human CD8 (clone UCHT4, 65204-1-Ig) labeled with FlexAble CoraLite® Plus 405 Kit (KFA046).

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-PAF49 labeled with FlexAble CoraLite® Plus 488 Kit (KFA041, green), anti-Tubulin (66240-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA042, yellow), anti-Lamin labeled with FlexAble CoraLite® Plus 647 Kit (KFA043, magenta) and DAPI (blue). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Lamin labeled with FlexAble CoraLite® Plus 488 Kit (KFA041, green), anti-GNL3 (67169-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA042, red), anti-Tom20 (11802-1-AP) labeled with FlexAble CoraLite® Plus 647 Kit (KFA003, magenta) and DAPI (blue). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Tubulin (66240-1-Ig) labeled with FlexAble CoraLite® Plus 488 Kit (KFA041, green), anti-Lamin labeled with FlexAble CoraLite® Plus 647 Kit (KFA043, magenta), CoraLite® 594-conjugated GNL3 antibody (CL594-67169) and DAPI (blue). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-GNL3 (67169-1-Ig) labeled with FlexAble CoraLite® Plus 488 Kit (KFA041, green), mouse anti-Lamin and anti-mouse IgG secondary antibody Alexa Fluor® 568 (red), rabbit anti-COXIV (11242-1-AP) and anti-rabbit IgG secondary antibody Alexa Fluor® 647 (cyan) and DAPI (blue). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Tubulin (66240-1-Ig) labeled with FlexAble CoraLite® Plus 488 Kit (KFA041, green). Cell nuclei are in blue. Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD45 (clone HI30, 65109-1-Ig) labeled with FlexAble CoraLite® Plus 750 Kit (KFA024) and anti-human CD3 (clone OKT3, 65133-1-Ig) labeled with FlexAble CoraLite® Plus 488 Kit (KFA041).

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite® Plus 7505 Kit (KFA024) and anti-human CD8 (clone UCHT4, 65204-1-Ig) labeled with FlexAble CoraLite® Plus 488 Kit (KFA041).

WB of HeLa and HepG2 cell lysates: HeLa and HepG2 cell lysates were detected with anti-HSP90 (60318-1-Ig) labeled with FlexAble CoraLite® Plus 488 Kit (KFA041, green), anti-CACYBP (68161-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA042, red) and anti-TUFM (67802-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA043, blue).

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Lamin labeled with FlexAble CoraLite® Plus 488 Kit (KFA041, green), anti-GNL3 (67169-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA042, red), anti-Tom20 (11802-1-AP) labeled with FlexAble CoraLite® Plus 647 Kit (KFA003, magenta) and DAPI (blue). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Vimentin labeled with FlexAble CoraLite® Plus 405 Kit (KFA026, cyan), anti-HSP60 (66041-1-Ig) labeled with FlexAble CoraLite® Plus 488 Kit (KFA021, green), anti-Tubulin (66240-1-lg) labeled with FlexAble CoraLite® Plus 555 Kit (KFA042, red) and anti-HNRNPA2B1 (67445-1-lg) labeled with FlexAble CoraLite® Plus 647 Kit (KFA043, magenta). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-TOM70 (14528-1-AP) labeled with FlexAble CoraLite® Plus 488 Kit (KFA001, green), anti-GNL3 (67169-1-lg) labeled with FlexAble CoraLite® Plus 555 Kit (KFA042, red) and anti-Actin labeled with FlexAble CoraLite® Plus 647 Kit (KFA043, magenta). Cell nuclei are in cyan. Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-PAF49 labeled with FlexAble CoraLite® Plus 488 Kit (KFA041, green), anti-Tubulin (66240-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA042, yellow), anti-Lamin labeled with FlexAble CoraLite® Plus 647 Kit (KFA043, magenta) and DAPI (blue).  Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-GNL3 (67169-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA042, red) and DAPI (blue). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD45 (clone HI30, 65109-1-Ig) labeled with FlexAble CoraLite® Plus 405 Kit (KFA026) and anti-human CD3 (clone OKT3, 65133-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA042).

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite® Plus 405 Kit (KFA026) and anti-human CD8 (clone UCHT4, 65204-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA042).

WB of HeLa and HepG2 cell lysates: HeLa and HepG2 cell lysates were detected with anti-HSP90 (60318-1-Ig) labeled with FlexAble CoraLite® Plus 488 Kit (KFA041, green), anti-CACYBP (68161-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA042, red) and anti-TUFM (67802-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA043, blue).

WB of HeLa and HepG2 cell lysates: HeLa and HepG2 cell lysates were detected with anti-TUFM (67802-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA042, red) and anti-CACYBP (68161-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA043, blue). 

Immunofluorescence of A431 cells: PFA-fixed cells were stained with anti-GNL3 (67169-1-Ig) labeled with FlexAble CoraLite® Plus 594 Kit (KFA049, orange) and DAPI (blue). ​ Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Flow cytometry of PBMCs. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-CD45RO (clone UCHL1, 65150-1-Ig) labeled with FlexAble CoraLite® Plus 594 Kit (KFA049) and co-stained anti-CD45RA (CL750-65226) antibody. Cells are not fixed.

Flow cytometry of PBMCs. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-CD3 (clone OKT3, 65133-1-Ig) labeled with FlexAble CoraLite® Plus 594 Kit (KFA049) and anti-CD4 (CL750-65134). Cells are not fixed.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Tubulin (66240-1-Ig) labeled with FlexAble CoraLite® Plus 488 Kit (KFA041, green), anti-Lamin labeled with FlexAble CoraLite® Plus 647 Kit (KFA043, magenta), CoraLite® 594-conjugated GNL3 antibody (CL594-67169) and DAPI (blue). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-GNL3 (67169-1-lg) labeled with FlexAble CoraLite® Plus 555 Kit (KFA042, red) and anti-Actin labeled with FlexAble CoraLite® Plus 647 Kit (KFA043, magenta). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-TOM70 (14528-1-AP) labeled with FlexAble CoraLite® Plus 488 Kit (KFA001, green), anti-GNL3 (67169-1-lg) labeled with FlexAble CoraLite® Plus 555 Kit (KFA042, red) and anti-Actin labeled with FlexAble CoraLite® Plus 647 Kit (KFA043, magenta). Cell nuclei are in cyan. Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-PAF49 labeled with FlexAble CoraLite® Plus 488 Kit (KFA041, green), anti-Tubulin (66240-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA042, yellow), anti-Lamin labeled with FlexAble CoraLite® Plus 647 Kit (KFA043, magenta) and DAPI (blue).  Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Actin labeled with FlexAble CoraLite® Plus 647 Kit (KFA043, magenta) and DAPI (blue). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD45 (clone HI30, 65109-1-Ig) labeled with FlexAble CoraLite® Plus 405 Kit (KFA026) and anti-human CD3 (clone OKT3, 65133-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA043).

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite® Plus 405 Kit (KFA026) and anti-human CD8 (clone UCHT4, 65204-1-Ig) labeled with FlexAble CoraLite® Plus 6475 Kit (KFA043).

WB of HeLa and HepG2 cell lysates: HeLa and HepG2 cell lysates were detected with anti-HSP90 (60318-1-Ig) labeled with FlexAble CoraLite® Plus 488 Kit (KFA041, green), anti-CACYBP (68161-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA042, red) and anti-TUFM (67802-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA043, blue).

WB of HeLa and HepG2 cell lysates: HeLa and HepG2 cell lysates were detected with anti-TUFM (67802-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA042, red) and anti-CACYBP (68161-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA043, blue). 

PFA-fixed HeLa cells were stained with anti-GNL3 (67169-1-Ig) labeled with FlexAble CoraLite® Plus 405 Kit (KFA046, cyan) and anti-Tubulin (66240-1-Ig) labeled with FlexAble CoraLite® Plus 750 Kit (KFA044, grey). Epifluorescence images were acquired with a 20x objective and post-processed.​

PFA-fixed HeLa cells were stained with anti-Tubulin (66240-1-Ig) labeled with FlexAble CoraLite® Plus 750 Kit (KFA044, grey) and Sytox Deep Red (cell nuclei, magenta). Epifluorescence images were acquired with a 20x objective and post-processed.

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD45 (clone HI30, 65109-1-Ig) labeled with FlexAble CoraLite® Plus 405 Kit (KFA026) and anti-human CD3 (clone OKT3, 65133-1-Ig) labeled with FlexAble CoraLite® Plus 750 Kit (KFA044).

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite® Plus 405 Kit (KFA026) and anti-human CD8 (clone UCHT4, 65204-1-Ig) labeled with FlexAble CoraLite® Plus 750 Kit (KFA044).​

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite® Plus 750 Kit (KFA024) and anti-human CD8 (clone UCHT4, 65204-1-Ig) labeled with FlexAble FITC Plus Kit (KFA048).

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD45 (clone HI30, 65109-1-Ig) labeled with FlexAble CoraLite® Plus 750 Kit (KFA024) and anti-human CD3 (clone OKT3, 65133-1-Ig) labeled with FlexAble FITC Plus Kit (KFA048).​

HeLa cells were immunostained with mouse IgG2a anti-Tubulin beta antibody (cat# 66240-1-Ig), labeled with FlexAble 2.0 CoraLite® Plus 405 Kit (KFA546, cyan). Cell nuclei are in red. Epifluorescence images were acquired with a 20x objective and post-processed.

Immunofluorescence of Rat brain: FFPE Rat brain sections were stained with anti-MAP2 (17490-1-AP) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA503, magenta), anti-GFAP (60190-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA541, green), anti-TDP-43 (67345-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA542,organge) and DAPI (blue).

Immunofluorescence of Rat brain: FFPE Rat brain sections were stained with anti-MAP2 (17490-1-AP) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA503, magenta), anti-GFAP (60190-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA541, green), anti-TDP-43 (67345-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA542,organge) and DAPI (blue).

Immunofluorescence of Rat brain: FFPE Rat brain sections were stained with anti-MAP2 (17490-1-AP) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA502, orange), anti-GFAP (60190-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA543, magenta), anti-TDP-43 (67345-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA541,green) and DAPI (blue).

Immunofluorescence of Rat brain: FFPE Rat brain sections were stained with anti-MAP2 (17490-1-AP) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA502, orange), anti-GFAP (60190-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA543, magenta), anti-TDP-43 (67345-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA541,green) and DAPI (blue).

Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG2a anti-GNL3 antibody (67169-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA541, green), mouse IgG2a anti-Tubulin antibody (66240-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA542, red), mouse IgG2a anti-PAF49 antibody labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA543, magenta) and mouse IgG2a anti-Clec12a antibody labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA544, cyan). Cell nuclei were stained with DAPI (blue).  Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG2a anti-GNL3 antibody (67169-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA541, green), mouse IgG2a anti-Tubulin antibody (66240-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA542, red), mouse IgG2a anti-PAF49 antibody labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA543, magenta) and mouse IgG2a anti-Clec12a antibody labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA544, cyan). Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG2a anti-GNL3 antibody (67169-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA541, green). Cell nuclei were stained with DAPI (blue).  Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

1X10^6 human PBMCs were surface co-stained with Anti-Human CD3 (65133-1-Ig, Clone:OKT3) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA541), and Anti-Human CD4 (65143-1-Ig, Clone:RPA-T4) labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA524). Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface co-stained with Anti-Human CD45RO (65150-1-Ig, Clone:UCHL1) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA541), and Anti-Human CD45RA (65226-1-Ig, Clone:F8-11-13) labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA524). Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface co-stained with CoraLite® Plus 405 Anti-Human CD3 (CL405-65151, Clone: UCHT1), Anti-Human CD8 (65135-1-Ig, Clone:OKT8) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA541), and Anti-Human CD4 (65143-1-Ig, Clone:RPA-T4) labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA524). Cells were not fixed. CD3+ lymphocytes were gated.

Immunofluorescence of Rat brain: FFPE Rat brain sections were stained with anti-MAP2 (17490-1-AP) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA501, green), anti-GFAP (60190-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA542, orange), anti-TDP-43 (67345-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA543,magenta) and DAPI (blue).

Immunofluorescence of Rat brain: FFPE Rat brain sections were stained with anti-MAP2 (17490-1-AP) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA501, green), anti-GFAP (60190-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA542, orange), anti-TDP-43 (67345-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA543,magenta) and DAPI (blue).

Immunofluorescence of Rat brain: FFPE Rat brain sections were stained with anti-MAP2 (17490-1-AP) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA503, magenta), anti-GFAP (60190-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA541, green), anti-TDP-43 (67345-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA542,organge) and DAPI (blue).

Immunofluorescence of Rat brain: FFPE Rat brain sections were stained with anti-MAP2 (17490-1-AP) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA503, magenta), anti-GFAP (60190-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA541, green), anti-TDP-43 (67345-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA542,organge) and DAPI (blue).

Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG2a anti-GNL3 antibody (67169-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA541, green), mouse IgG2a anti-Tubulin antibody (66240-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA542, red), mouse IgG2a anti-PAF49 antibody labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA543, magenta) and mouse IgG2a anti-Clec12a antibody labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA544, cyan). Cell nuclei were stained with DAPI (blue).  Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG2a anti-GNL3 antibody (67169-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA541, green), mouse IgG2a anti-Tubulin antibody (66240-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA542, red), mouse IgG2a anti-PAF49 antibody labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA543, magenta) and mouse IgG2a anti-Clec12a antibody labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA544, cyan). Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG2a anti-Tubulin antibody (66240-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA542, green). Cell nuclei were stained with DAPI (blue).  Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

1X10^6 human PBMCs were surface co-stained with Anti-Human CD3 (65133-1-Ig, Clone:OKT3) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA542), and Anti-Human CD4 (65143-1-Ig, Clone:RPA-T4) labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA524). Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface co-stained with Anti-Human CD45RO (65150-1-Ig, Clone:UCHL1) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA542), and Anti-Human CD45RA (65226-1-Ig, Clone:F8-11-13) labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA524). Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface co-stained with CoraLite® Plus 405 Anti-Human CD3 (CL405-65151, Clone: UCHT1), Anti-Human CD8 (65135-1-Ig, Clone:OKT8) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA542), and Anti-Human CD4 (65143-1-Ig, Clone:RPA-T4) labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA524). Cells were not fixed. CD3+ lymphocytes were gated.

HeLa cells were immunostained with mouse IgG2a anti-Tubulin beta antibody (cat# 66240-1-Ig), labeled with FlexAble 2.0 CoraLite® Plus 594 Kit (KFA549, red). Cell nuclei are in cyan. Epifluorescence images were acquired with a 20x objective and post-processed.

Immunofluorescence of Rat brain: FFPE Rat brain sections were stained with anti-MAP2 (17490-1-AP) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA501, green), anti-GFAP (60190-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA542, orange), anti-TDP-43 (67345-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA543,magenta) and DAPI (blue).

Immunofluorescence of Rat brain: FFPE Rat brain sections were stained with anti-MAP2 (17490-1-AP) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA501, green), anti-GFAP (60190-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA542, orange), anti-TDP-43 (67345-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA543,magenta) and DAPI (blue).

Immunofluorescence of Rat brain: FFPE Rat brain sections were stained with anti-MAP2 (17490-1-AP) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA502, orange), anti-GFAP (60190-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA543, magenta), anti-TDP-43 (67345-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA541,green) and DAPI (blue).

Immunofluorescence of Rat brain: FFPE Rat brain sections were stained with anti-MAP2 (17490-1-AP) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA502, orange), anti-GFAP (60190-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA543, magenta), anti-TDP-43 (67345-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA541,green) and DAPI (blue).

Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG2a anti-GNL3 antibody (67169-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA541, green), mouse IgG2a anti-Tubulin antibody (66240-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA542, red), mouse IgG2a anti-PAF49 antibody labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA543, magenta) and mouse IgG2a anti-Clec12a antibody labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA544, cyan). Cell nuclei were stained with DAPI (blue).  Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG2a anti-GNL3 antibody (67169-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA541, green), mouse IgG2a anti-Tubulin antibody (66240-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA542, red), mouse IgG2a anti-PAF49 antibody labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA543, magenta) and mouse IgG2a anti-Clec12a antibody labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA544, cyan). Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG2a anti-PAF49 antibody labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA543, magenta). Cell nuclei were stained with DAPI (blue).  Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

1X10^6 human PBMCs were surface co-stained with Anti-Human CD3 (65133-1-Ig, Clone:OKT3) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA543), and Anti-Human CD4 (65143-1-Ig, Clone:RPA-T4) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA521). Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface co-stained with Anti-Human CD45RO (65150-1-Ig, Clone:UCHL1) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA543), and Anti-Human CD45RA (65226-1-Ig, Clone:F8-11-13) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA521). Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface co-stained with CoraLite® Plus 405 Anti-Human CD3 (CL405-65151, Clone: UCHT1), Anti-Human CD8 (65135-1-Ig, Clone:OKT8) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA543), and Anti-Human CD4 (65143-1-Ig, Clone:RPA-T4) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA521). Cells were not fixed. CD3+ lymphocytes were gated.

Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG2a anti-GNL3 antibody (67169-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA541, green), mouse IgG2a anti-Tubulin antibody (66240-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA542, red), mouse IgG2a anti-PAF49 antibody labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA543, magenta) and mouse IgG2a anti-Clec12a antibody labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA544, cyan). Cell nuclei were stained with DAPI (blue).  Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG2a anti-GNL3 antibody (67169-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA541, green), mouse IgG2a anti-Tubulin antibody (66240-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA542, red), mouse IgG2a anti-PAF49 antibody labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA543, magenta) and mouse IgG2a anti-Clec12a antibody labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA544, cyan). Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of Hela cells: PFA-fixed cells were stained with mouse IgG2a anti-Clec12a antibody labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA544, cyan). Cell nuclei were stained with DAPI (blue).  Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

1X10^6 human PBMCs were surface co-stained with Anti-Human CD3 (65133-1-Ig, Clone:OKT3) labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA544), and Anti-Human CD4 (65143-1-Ig, Clone:RPA-T4) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA521). Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface co-stained with Anti-Human CD45RO (65150-1-Ig, Clone:UCHL1) labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA544), and Anti-Human CD45RA (65226-1-Ig, Clone:F8-11-13) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA521). Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface co-stained with CoraLite® Plus 405 Anti-Human CD3 (CL405-65151, Clone: UCHT1), Anti-Human CD8 (65135-1-Ig, Clone:OKT8) labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA544), and Anti-Human CD4 (65143-1-Ig, Clone:RPA-T4) labeled with FlexAble 2.0  CoraLite® Plus 488 Kit (KFA521). Cells were not fixed. CD3+ lymphocytes were gated.