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29 product results for:

"alpha tubulin"

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Alpha Tubulin Recombinant antibody

KO/KD Validated

4 Publications

View More Images (4)

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Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Alpha Tubulin antibody (80762-1-RR, Clone: 1G7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat, Human

Applications

WB, IF, ELISA

CloneNo.

1G7

Sizes
-_- / -_-

Cat No. 80762-1-RR
×

The search results gallery shows a selection of our total image library
More validation images available on the product page

Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Alpha Tubulin antibody (80762-1-RR, Clone: 1G7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Various lysates were subjected to SDS PAGE followed by western blot with 80762-1-RR (Tubulin-Alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

WB result of Alpha Tubulin antibody (80762-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Alpha Tubulin transfected HEK-293 cells.

Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using Alpha Tubulin antibody (80762-1-RR, Clone: 1G7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Alpha Tubulin Polyclonal antibody

1327 Publications

Reviews (13)

View More Images (7)

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Various lysates were subjected to SDS PAGE followed by western blot with 11224-1-AP (alpha Tubulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

chicken, goat, hamster, human and More (7)

Applications

WB, IP, IF, FC, IHC, CoIP, ELISA

Sizes
-_- / -_-

Cat No. 11224-1-AP
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Various lysates were subjected to SDS PAGE followed by western blot with 11224-1-AP (alpha Tubulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Lymphatic endothelial cells were stained for CD31 (green, Cat. No CL488-66065, 1:200) and alpha tubulin (magenta, Cat. 11224-1-AP, 1:200) with DAPI as a counterstain for visualizing the nucleus (yellow). CD31 is an endothelial cell adhesion protein and was conjugated to CoraLite-488 fluorescent dye to allow for direct visualization. Alpha tubulin is a cytoskeletal protein important in cell division and migration and was visualized with an Alexa Fluor 568 secondary antibody. Cells were fixed with 4% paraformaldehyde. Image credit: @Immunofluorescence on Instagram

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Alpha Tubulin antibody (11224-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

1X10^6 HEK-293 cells were stained with 0.2ug alpha Tubulin antibody (11224-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). FITC-Goat anti-Rabbit IgG with dilution 1:100.

IP result of anti-Alpha Tubulin (IP:11224-1-AP, 3ug; Detection:11224-1-AP 1:1000) with HeLa cells lysate 5000ug.

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11224-1-AP (Alpha Tubulin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11224-1-AP (Alpha Tubulin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Alpha Tubulin Monoclonal antibody

KO/KD Validated

928 Publications

Reviews (10)

View More Images (11)

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Various lysates were subjected to SDS PAGE followed by western blot with 66031-1-Ig (Alpha Tubulin antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours.

Host/IsoType

Mouse/IgG2b

Reactivity

Canine, chicken, goat, human and More (8)

Applications

WB, IP, IF, FC, IHC, CoIP, ELISA

CloneNo.

1E4C11

Sizes
-_- / -_-

Cat No. 66031-1-Ig
×

The search results gallery shows a selection of our total image library
More validation images available on the product page

Various lysates were subjected to SDS PAGE followed by western blot with 66031-1-Ig (Alpha Tubulin antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours.

WB result of Alpha Tubulin antibody (66031-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Alpha Tubulin transfected HeLa cells.

Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11 ) at dilution of 1:1000 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), ZO-1 antibody (21773-1-AP, green).

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11 ) at dilution of 1:1000 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Histone H1.2 antibody (19649-1-AP, green).

Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66031-1-Ig(alpha Tubulin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66031-1-Ig (alpha Tubulin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IP result of anti-Alpha Tubulin (IP:66031-1-Ig, 5ug; Detection:11224-1-AP 1:1000) with HeLa cells lysate 2800ug.

1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Alpha Tubulin (66031-1-Ig, Clone:1E4C11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66031-1-Ig (Alpha Tubulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66031-1-Ig (Alpha Tubulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), ZO-1 antibody (21773-1-AP, red).
Alpha Tubulin Recombinant antibody, PBS Only (Detector)

KO/KD Validated

View More Images (5)

×
Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Alpha Tubulin antibody (80762-1-RR, Clone: 1G7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80762-1-PBS in a different storage buffer formulation.

Host/IsoType

Rabbit/IgG

Reactivity

mouse, rat, Human

Applications

WB, IF, Indirect ELISA, Cytometric bead array

CloneNo.

1G7

Sizes
-_- / -_-

Cat No. 80762-1-PBS
×

The search results gallery shows a selection of our total image library
More validation images available on the product page

Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Alpha Tubulin antibody (80762-1-RR, Clone: 1G7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80762-1-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 80762-1-RR (Tubulin-Alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80762-1-PBS in a different storage buffer formulation.

WB result of Alpha Tubulin antibody (80762-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Alpha Tubulin transfected HEK-293 cells. This data was developed using the same antibody clone with 80762-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using Alpha Tubulin antibody (80762-1-RR, Clone: 1G7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80762-1-PBS in a different storage buffer formulation.

Cytometric bead array standard curve of MP00551-1, Tubulin-Alpha Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 80740-1-PBS. Detection antibody: 80762-1-PBS. Standard:Ag18034. Range: 0.098-50 ng/mL.
HRP-conjugated Alpha Tubulin Monoclonal antibody

113 Publications

Enlarge Image

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Western blot analysis of alpha-tubulin in various tissues and cell lines using Proteintech antibody HRP-60031 at a dilution of 1:5000.

Host/IsoType

Mouse/IgG2b

Reactivity

human, mouse, rat, human

Applications

WB, CoIP

Conjugate

HRP

CloneNo.

1E4C11

Sizes
-_- / -_-

Cat No. HRP-66031
×

The search results gallery shows a selection of our total image library
More validation images available on the product page

Western blot analysis of alpha-tubulin in various tissues and cell lines using Proteintech antibody HRP-60031 at a dilution of 1:5000.
Alpha Tubulin Polyclonal antibody

9 Publications

View More Images (3)

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mouse brain tissue were subjected to SDS PAGE followed by western blot with 14555-1-AP (alpha Tubulin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat, human

Applications

WB, IHC, IF, ELISA

Sizes
-_- / -_-

Cat No. 14555-1-AP
×

The search results gallery shows a selection of our total image library
More validation images available on the product page

mouse brain tissue were subjected to SDS PAGE followed by western blot with 14555-1-AP (alpha Tubulin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of HepG2 cells using 14555-1-AP (alpha Tubulin antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14555-1-AP (Alpha Tubulin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
CoraLite® Plus 488-conjugated Alpha Tubulin Monoclonal antibody

3 Publications

View More Images (4)

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E14.5 FFPE mouse embryo section stained for smooth muscle actin (red, Cat. No 80008-1-RR) and alpha tubulin (green, Cat. CL488-66031). The alpha tubulin was conjugated to CoraLite-488 fluorescent dye. In red, you can see muscle tissue clearly stained including the tongue, heart, and arteries. Nerves and neurons are stained green since they are rich in tubulin. Image credit: @Immunofluorescence on Instagram

Host/IsoType

Mouse/IgG2b

Reactivity

canine, human, mouse, rat

Applications

WB, IF, FC (Intra)

Conjugate

CoraLite® Plus 488

CloneNo.

1E4C11

Sizes
-_- / -_-

Cat No. CL488-66031
×

The search results gallery shows a selection of our total image library
More validation images available on the product page

E14.5 FFPE mouse embryo section stained for smooth muscle actin (red, Cat. No 80008-1-RR) and alpha tubulin (green, Cat. CL488-66031). The alpha tubulin was conjugated to CoraLite-488 fluorescent dye. In red, you can see muscle tissue clearly stained including the tongue, heart, and arteries. Nerves and neurons are stained green since they are rich in tubulin. Image credit: @Immunofluorescence on Instagram

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using the CoraLite® Plus 488-conjugated version of this antibody, CL488-66031 (alpha Tubulin antibody), at dilution of 1:100.

Various lysates were subjected to SDS PAGE followed by western blot with CL488-66031 (Alpha Tubulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Alpha Tubulin (CL488-66031, Clone:1E4C11) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
CoraLite®594-conjugated Alpha Tubulin Monoclonal antibody

3 Publications

View More Images (4)

×
Various lysates were subjected to SDS PAGE followed by western blot with CL594-66031 (Alpha Tubulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Host/IsoType

Mouse/IgG2b

Reactivity

Canine, human, mouse, rat and More (1)

Applications

WB, IF, FC

Conjugate

CoraLite®594

CloneNo.

1E4C11

Sizes
-_- / -_-

Cat No. CL594-66031
×

The search results gallery shows a selection of our total image library
More validation images available on the product page

Various lysates were subjected to SDS PAGE followed by western blot with CL594-66031 (Alpha Tubulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-66031 (alpha Tubulin antibody) at dilution of 1:100.

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using the CoraLite®594-conjugated version of this antibody, CL594-66031 (alpha Tubulin antibody), at dilution of 1:100.

1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human Alpha Tubulin (CL594-66031, Clone:1E4C11) (red), or 0.8 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Biotin-conjugated Alpha Tubulin Monoclonal antibody

1 Publications

View More Images (2)

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Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using Biotin-66031 (alpha Tubulin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Host/IsoType

Mouse/IgG2b

Reactivity

Canine, mouse, rat, human

Applications

WB, IHC

Conjugate

Biotin

CloneNo.

1E4C11

Sizes
-_- / -_-

Cat No. Biotin-66031
×

The search results gallery shows a selection of our total image library
More validation images available on the product page

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using Biotin-66031 (alpha Tubulin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using Biotin-66031 (alpha Tubulin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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29 product results for:

"alpha tubulin"

Alpha Tubulin Recombinant antibody

-_- / -_-

Alpha Tubulin Polyclonal antibody

-_- / -_-

Alpha Tubulin Monoclonal antibody

-_- / -_-

Alpha Tubulin Recombinant antibody, PBS Only (Detector)

-_- / -_-

HRP-conjugated Alpha Tubulin Monoclonal antibody

-_- / -_-

Alpha Tubulin Polyclonal antibody

-_- / -_-

CoraLite® Plus 488-conjugated Alpha Tubulin Monoclonal antibody

-_- / -_-

CoraLite®594-conjugated Alpha Tubulin Monoclonal antibody

-_- / -_-

Biotin-conjugated Alpha Tubulin Monoclonal antibody

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